D-GalN/LPS诱导的急性肝衰竭小鼠肝组织cGAS-STING通路基因水平变化*

doi:10.3969/j.issn.1672-5069.2026.04.002

Abstract

Abstract: Objective The aim of this experiment was to investigate the effect of cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS)/stimulator of interferon gene (STING) pathway on ALF in vivo. Methods Thirty C57BL/6J mice were randomly assigned into control, model and intervention group, with 10 mice in each. Model was established by intraperitoneal injection of D-GalN/LPS, intervened by specific inhibitor C-176 and gene RNA was detected by qRT-PCR. Results Compared to in the model group, the C-176 intervention group exhibited a significant prolonged survival time (P<0.05); extensive hepatocellular necrosis and disordered architecture was found in the model group, whereas the liver tissue structure in the C-176 intervention group appeared more organized with reduced necrotic areas; immunohistochemistry and gene analysis demonstrated that the protein expression and mRNA levels of key components in the STING signaling pathway were significantly upregulated in the model group, but were notably downregulated in the C-176 intervention group; furthermore, IFN-Ⅰ, IL-1β, IL-6, TNF-α and F4/80, CD4, CD8, Gr1, NK1.1 and CD11c significantly decreased in the STING intervention group compared to the those in model group (P<0.05). Conclusion The STING pathway is involved in the inflammatory storm associated with ALF occurrence, and inhibition of this signaling pathway might alleviate immune-mediated liver injury in ALF.

Key words: Acute liver failure, cyclic guanosine monophosphate-adenosine monophosphate synthase /stimulator of interferon gene pathway, Cytokine storm, Mice

Lin Jing, Jia Jihui, Duan Zhongping, et al. cGAS-STING pathway is involved in regulating the development of D-GalN/LPS-induced acute liver failure in mice[J]. Journal of Practical Hepatology, 2026, 29(4): 485-488.

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cGAS-STING pathway is involved in regulating the development of D-GalN/LPS-induced acute liver failure in mice

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