Abstract: Objective Primary biliary cholangitis (PBC) is a common autoimmune disease all over the world. The aim of this study was to investigate prevalence of serum autoantibodies and clinic features in patients with PBC. Methods A retrospective analysis was performed on 230 patients with PBC. All clinic characteristic and serum autoantibodies including Anti-Sm, RNP, SSA60, SSA52, SSB, CENP-B, dsDNA, antihistone antibodies, AMA-M2, Sp100, gp210 and ANua, were assayed. Other autoimmune diseases, such as Sjogren syndrome (SS), systemic lupus erythematosus (SLE) were recorded. Results Out of the 230 patients with PBC, the prevalence of serum AMA-M2, anti-gp210 and anti-Sp100 were positive in 167(72.6%), 101(43.9%), and 39(17%) patients; serum ANA was positive in 178(77.4%) patients; serum anti-SSA and anti-CENP-B were positive in 37.4% and 20.0% in spite of positive serum autoantibodies above; SS and SLE were found in 54 and 21 patients (23.5% and 9.1%); anti-gp210 and anti-SSA52 had a higher positive rates in 74 patients with decompensated liver functions (63.5% vs. 34.6%,P <0.05 and 44.6% vs. 29.5%, respectively, P <0.05) as compared to those in 156 patients with compensated liver functions. Conclusion Serum AMA-M2, anti-GP210 and anti-Sp100 are still key diagnostic index, and serum anti-SSA52 and anti-CENP-B have relatively high positive rate in patients with PBC. We also found that the patients with positive anti-gp210 and anti-SSA52 might have a severe liver disease.

Key words: Primary biliary cholangitis, Autoantibody, Diagnosis