Abstract: Objective To observe the effect of barbata on transforming growth factor-β1 and tumor necrosis factor-α expression in rats with CCl4-induced liver fibrosis. Methods The model of CCl4 induced liver fibrosis in rats were established. The serum and liver tissue TGF-β1 and TNF-α were detected by immunohistochemistry and ELISA. Enzyme-linked immunosorbent dot technology（ELISPOT）was used to detect the single-cell secretion of TNF-α. Result TNF-α and TGF-β1 were negative in the normal control group and were obviously positive in model group;Barbata inhibited the expression of both TNF-αand TGF-β1in liver tissues and in sera. Conclusion Barbata has an antifibrotic effect by inhibition of cytokines in the liver.