体外感染实验探讨丙型肝炎病毒的ADE分子机制

实用肝脏病杂志 ›› 2010, Vol. 13 ›› Issue (1): 4-8.

体外感染实验探讨丙型肝炎病毒的ADE分子机制

聂青和, 罗新栋, 黄晓峰, 张亚飞, 程勇前

710038 西安市第四军医大学唐都医院感染病科/全军感染病诊疗中心（聂青和,罗新栋,张亚飞,程勇前）; 电子显微镜室（黄晓峰）

收稿日期:2009-07-28 出版日期:2010-02-10 发布日期:2016-04-18
作者简介:聂青和男,48岁,教授、主任医师、博士生导师。主要研究方向：丙型肝炎及肝纤维化。E-mail：nieqinghe@163.com
基金资助:
国家自然科学基金资助项目（No：30771891）

Experimental study of in vitro infection of human trophoblastic cells with hepatitis C virus

NIE Qinghe, LUO Xindong, HUANG Xiaofeng, et al.

The Center of Diagnosis and Treatment for Infectious Diseases of PLA,Tangdu Hospital,The Fourth Military Medical University,Xi'an 710038

Received:2009-07-28 Online:2010-02-10 Published:2016-04-18

摘要/Abstract

摘要： 目的研究丙型肝炎病毒（HCV）在感染人滋养层细胞的过程中是否存在抗体依赖性感染增强（ADE）作用,以探讨HCV母婴传播的分子机制。方法将HCV阳性血清以4种不同方式感染人滋养层细胞,以免疫电镜观察HCV病毒颗粒在滋养层细胞中的表达,应用RT-PCR法、免疫组化法检测细胞内外的HCV RNA正链、负链,HCV NS5、NS3及C区抗原。结果在滋养层细胞胞浆内发现了HCV病毒颗粒,HCV RNA正链、负链,HCV NS5、NS3、C区抗原仅在全血清感染细胞内或上清中间断测得。结论 HCV可以在滋养层细胞中复制。HCV感染滋养层细胞的过程中存在ADE机制,抗体和补体均参与了HCV的跨膜转运过程。

关键词: 丙型肝炎病毒, 人滋养层细胞, 体外感染

Abstract: Objective To assess the antibody-dependent enhancement of infection（ADE） when the cultured trophoblastic cells were infected by hepatitis C virus（HCV）. Methods We incubated the cytotrophoblasts with HCV by four different methods. Under the immunoelectron microscopy,we observed the HCV viral particles. RT-PCR method and immunohistochemistry were employed to assay the expressions of HCV NS5 and NS3 and HCV-C antigen and HCV RNA in cells or supernatant. Results The HCV viral particles were observed in the cultured cytotrophoblasts. The HCV RNA and reverse RNA and HCV NS5 and NS3 and HCV-C antigen were only intermittently detected in the culture medium or the cells incubated with HCV RNA positive serum. Conclusion The HCV viral particles can replicate in the cultured cytotrophoblasts. The ADE plays an important role when HCV viral particles enter the cultured cytotrophoblasts. Anti-HCV and/or complement probably enhanced the infection of viral particles.

Key words: Hepatitis C virus, Cytotrophoblasts, In vitro

聂青和, 罗新栋, 黄晓峰, 张亚飞, 程勇前. 体外感染实验探讨丙型肝炎病毒的ADE分子机制[J]. 实用肝脏病杂志, 2010, 13(1): 4-8.

NIE Qinghe, LUO Xindong, HUANG Xiaofeng, et al.. Experimental study of in vitro infection of human trophoblastic cells with hepatitis C virus[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2010, 13(1): 4-8.

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