LINE-1 ORF-1p过表达对肝癌细胞株SMMC7721增殖和锚定非依赖性生长的影响*

doi:10.3969/j.issn.1672-5069.2011.05.002

实用肝脏病杂志 ›› 2011, Vol. 14 ›› Issue (5): 323-326.doi: 10.3969/j.issn.1672-5069.2011.05.002

LINE-1 ORF-1p过表达对肝癌细胞株SMMC7721增殖和锚定非依赖性生长的影响^*

高旭东,陆荫英,冯帆,王春平,王鈜,常秀娟,杨永平

100039 北京市解放军第302医院肝脏肿瘤诊疗与研究中心（高旭东，陆荫英，王春平，王鈜，常秀娟，杨永平）；解放军军事医学科学院毒物药物研究所（冯帆）

收稿日期:2011-06-08 出版日期:2011-10-15 发布日期:2016-04-14
通讯作者: 杨永平，E-mail:yongpingyang@hotmail.com
作者简介:高旭东男，36岁，医学硕士，主治医师。主要从事肝癌的基础与临床研究。
基金资助:
国家科技重大专项课题（2008ZX100022018）；首都医学发展基金课题（2009-2041）

Effects of LINE-1 ORF-1p overexpression on proliferation and anchor-independent growth of SMMC7721 hepatoma cell line

GAO Xudong，LU Yinying，FENG Fan，et al.

Center for Liver Cancer，302nd Hospital of PLA，Beijing 100039，China

Received:2011-06-08 Online:2011-10-15 Published:2016-04-14

摘要/Abstract

摘要： 目的研究逆转座子L1编码蛋白ORF-1p的表达对肝癌细胞株SMMC7721细胞增殖的影响。方法利用带Flag标签的L1 ORF1表达载体，转染SMMC7721细胞；采用Western blot法检测反转座子L1 ORF1编码蛋白ORF-1p的表达；采用软琼脂成集落实验和相关报告基因检测ORF-1p表达对肝癌细胞p53、p15和p21活性的影响。结果在SMMC7721细胞中，Flag-ORF-1p 表达能够显著促进该细胞的增殖（P<0.05），增强该细胞的锚定非依赖性生长（P<0.05），降低p53、p15和p21报告基因的活性。结论 L1基因编码蛋白ORF-1p能够促进肝癌细胞的生长、浸润以及肿瘤的形成。

关键词: 肝癌细胞株SMMC7721, 逆转座子L1, 开放读码框-1p, 蛋白质转移吸印技术

Abstract: Objective To investigate the effects of L1 encoded ORF-1p on the proliferation of SMMC7721 cells. Methods Recombinant expression vector pcDNA3.0-Flag-ORF1 was used to transfect SMMC7721 cells;Expression of ORF1p in the transfected cells was detected by Western blotting;Proliferation and anchor-independent growth of the cells transfected with Flag-ORF1p vector were detected by MTT assays and soft-agar anchored colony formation assays;Transcriptional activity of p15，p21 and p53 reporter genes was analyzed by luciferase reporter system. Results Flag-ORF-1p could enhance the proliferation and anchor-independent growth ability of SMMC7721 cells;It also reduced the transcriptional activity of p15，p21 and p53 luciferase report genes. Conclusion ORF-1p of L1 might enhance the SMMC7721 cell proliferation and anchor-independent growth through regulation of some genes.

Key words: SMMC7721 cells, Retrotransposon LINE-1, Open reading frame-1p, Carcinogenesis

高旭东,陆荫英,冯帆,王春平,王鈜,常秀娟,杨永平. LINE-1 ORF-1p过表达对肝癌细胞株SMMC7721增殖和锚定非依赖性生长的影响^*[J]. 实用肝脏病杂志, 2011, 14(5): 323-326.

GAO Xudong，LU Yinying，FENG Fan，et al.. Effects of LINE-1 ORF-1p overexpression on proliferation and anchor-independent growth of SMMC7721 hepatoma cell line[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2011, 14(5): 323-326.

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LINE-1 ORF-1p过表达对肝癌细胞株SMMC7721增殖和锚定非依赖性生长的影响^*

Effects of LINE-1 ORF-1p overexpression on proliferation and anchor-independent growth of SMMC7721 hepatoma cell line

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