七氟醚对脓毒症大鼠肝损伤的保护作用及其作用机制研究*

doi:10.3969/j.issn.1672-5069.2019.05.007

摘要/Abstract

摘要： 目的探讨应用七氟醚预麻醉对脓毒症大鼠肝损伤的保护作用及其作用机制。方法将60只SD大鼠随机分为假手术组、脓毒症组和七氟醚预处理组,采取盲肠结扎穿孔(CLP)法建立脓毒症动物模型。采用Western bloting法检测肝组织淋巴细胞功能相关抗原-1（LFA-1)和巨噬细胞1抗原（Mac-1）表达。结果肝组织病理学检查显示,脓毒症组大鼠肝组织可见有明显的炎性细胞浸润,肝小叶肝索排列紊乱,肝细胞水肿明显,而七氟醚组肝组织肝索排列较为整齐,炎性细胞浸润较脓毒症组大鼠明显减少;脓毒症组动物血清AST、ALT和TBIL水平分别为（156.8±14.7） U/L、（75.6±7.5） U/L和（4.5±0.6） μmol/L,而七氟醚预处理组则分别为（100.5±7.9） U/L、（61.5±10.1） U/L和（3.2±0.5） μmol/L,各指标显著改善（P<0.05）;Western bloting检测结果显示,脓毒症组肝组织LFA-1和MAC-1相对表达水平分别为（1.2±0.2）和（1.0±0.2）,而七氟醚预处理组则分别为（0.7±0.2）和（0.6±0.1）,显著减弱（P<0.05）。结论给予实验动物七氟醚预处理麻醉可减轻脓毒症大鼠肝损伤,可能与减弱了肝组织LFA-1和MAC-1表达水平有关,值得进一步研究。

关键词: 脓毒症, 七氟醚, 麻醉, 淋巴细胞功能相关抗原-1, 巨噬细胞1抗原, 大鼠

Abstract: Objective To explore the protective effect and its mechanism of sevoflurane on sepsis-induced liver injury in rats. Methods Sixty SD rats were randomly divided into t sham operation,sepsis group and sevoflurane pretreatment group with 20 in each. In the sham operation group,the rats were only treated with open and closed abdominal suture,the sepsis model were established by cecal ligation perforation （CLP） and the animals in sevoflurane group were pretreated with sevoflurane anaesthesia before the establishment of CLP sepsis. The pathological changes of liver tissues were observed,and hepatic expression of lymphocyte function-associated antigen-1 （LFA-1） and macrophage-1 antigen （Mac-1） were detected by Western bloting. Results The pathological examination showed that the obvious inflammatory cell infiltration and hepatic cords arranged disorderly in model animals,while this kind of deformed morphology improved greatly in sevoflurane pretreatment group;serum AST,ALT and bilirubin levels in sepsis group were（156.8±14.7） U/L,（75.6±7.5） U/L and （4.5±0.6） μmol/L,while they decreased greatly in sevoflurane pretreatment group [（100.5±7.9） U/L,（61.5±10.1） U/L and （3.2±0.5） μmol/L,respectively,P<0.05];the Western bloting detection results demonstrated that the relative expression of hepatic LFA-1 and MAC-1 in sepsis animals were （1.2±0.2） and （1.0±0.2）,while they decreased greatly in sevoflurane pretreatment group[（0.7±0.2） and（0.6±0.1）,respectively,P<0.05]. Conclusion The application of sevoflurane for pretreatment anaesthesia might have a protective effect on sepsis-induced liver injury in rats,which is related to the down-regulation of hepatic LFA-1 and MAC-1 expression.

Key words: Sepsis, Sevoflurane, Anaesthesia, Lymphocyte function-associated antigen-1, Macrophage-1 antigen, Rats

石小龙, 杜睿, 秦革萍, 薛华燕, 张宏鑫. 七氟醚对脓毒症大鼠肝损伤的保护作用及其作用机制研究*[J]. 实用肝脏病杂志, 2019, 22(5): 640-643.

Shi Xiaolong, Du Rui, Qin Geping, et al. Protective effect of sevoflurane on sepsis-induced liver injury in rats[J]. Journal of Practical Hepatology, 2019, 22(5): 640-643.

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