慢加急性乙型肝炎肝衰竭患者外周血IFN-γ基因多态性分析及其临床意义探讨*

doi:10.3969/j.issn.1672-5069.2018.06.016

实用肝脏病杂志 ›› 2018, Vol. 21 ›› Issue (6): 890-894.doi: 10.3969/j.issn.1672-5069.2018.06.016

慢加急性乙型肝炎肝衰竭患者外周血IFN-γ基因多态性分析及其临床意义探讨^*

王燕, 吴琦琦, 庄小芳, 马燕, 郭峰, 王晓波, 王晓忠

830000乌鲁木齐市新疆医科大学附属中医医院肝病科

收稿日期:2017-09-22 出版日期:2018-11-10 发布日期:2018-12-25
通讯作者: 王晓忠,E-mail: wxz125@sina.com
作者简介:王燕,女,41岁,博士研究生,副主任医师。主要从事肝胆相关疾病中西医治疗。E-mail:112200141@qq.com
基金资助:
*国家中医药管理局国家中医临床研究基地业务建设科研专项课题（编号：JDZX2012064）

Polymorphism of blood IFN-γ gene in patients with hepatitis B induced acute-on-chronic liver failure

Wang Yan, Wu Qiqi, Zhuang Xiaofang, et al.

Department of Liver Diseases,Affiliated Chinese Medicine Hospital,Xinjiang Medical University,Urumqi 830000,Xinjiang Uygur Autonomous Region,China

Received:2017-09-22 Online:2018-11-10 Published:2018-12-25

摘要/Abstract

摘要： 目的探讨乙型肝炎病毒感染引起的慢加急性肝衰竭患者血IFN-γ基因多态性。方法采用单核苷酸多态性（SNP）技术检测51例ACLF患者和50例健康人血IFN-γ基因内含子+874 位点T/A 和+2109位点A/G单核苷酸多态性。结果 ACLF患者+874位点TA+AA基因型频率（54.9%）显著高于健康人（24.0%）,A等位基因频率（38.2%）显著高于健康人（22.0%,P<0.05）;ACLF组+2109位点AG+GG型基因型频率（51.0%）显著高于健康人（26.0%）,G等位基因（32.4%）显著高于健康人（16.0%,P<0.05）;在3 m末,28例生存与23例死亡的ACLF患者+874、+2109位点等位基因和基因型频率分布无显著性差异（P>0.05）。结论 IFN-γ基因+874 位点A等位基因和基因型、+2109位点G等位基因和基因型是ACLF的遗传易感基因。

关键词: 慢加急性肝衰竭, 乙型肝炎, 干扰素-γ, 基因多态性

Abstract: Objective To investigate blood IFN-γ gene polymorphism in patients with hepatitis B-induced acute-on-chronic liver failure （ACLF）. Method Single nucleotide polymorphism （SNP） technique was applied to detect blood single nucleotide polymorphism of IFN-γ gene +874T/A and +2109 A/G in 51 patients with ACLF and 50 healthy controls. Results The frequency of +874 locus TA+AA genotype in patients with ACLF was 54.9%,much higher than 24.0% in the control（P<0.05）,and A allel was 38.2%,also much higher than 22.0% in the control（P<0.05）;the frequency of +2109 locus AG+GG genotype in patients with ACLF was 51.0%,much higher than 26.0% in the control（P<0.05）,and G allel was 32.4%,much higher than 16.0% in the control（P<0.05）;there was no significant difference as respect to the frequencies of +874 and +2109 allele and genotype between 28 survivals and 23 dead（P>0.05） at the end of 3 month follow-up. Conclusion IFN-γ gene +874 locus A allel and its genotype and +2109 locus G allel and its genotype might be the genetic susceptibility genes for the occurrence of ACLF.

Key words: Acute-on-chronic liver failure, Hepatitis B, IFN-γ, Gene polymorphism

王燕, 吴琦琦, 庄小芳, 马燕, 郭峰, 王晓波, 王晓忠. 慢加急性乙型肝炎肝衰竭患者外周血IFN-γ基因多态性分析及其临床意义探讨^*[J]. 实用肝脏病杂志, 2018, 21(6): 890-894.

Wang Yan, Wu Qiqi, Zhuang Xiaofang, et al.. Polymorphism of blood IFN-γ gene in patients with hepatitis B induced acute-on-chronic liver failure[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(6): 890-894.

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慢加急性乙型肝炎肝衰竭患者外周血IFN-γ基因多态性分析及其临床意义探讨^*

Polymorphism of blood IFN-γ gene in patients with hepatitis B induced acute-on-chronic liver failure

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