乙型肝炎病毒相关性肝癌组织维生素D受体表达情况分析

doi:10.3969/j.issn.1672-5069.2018.02.023

实用肝脏病杂志 ›› 2018, Vol. 21 ›› Issue (2): 249-252.doi: 10.3969/j.issn.1672-5069.2018.02.023

乙型肝炎病毒相关性肝癌组织维生素D受体表达情况分析

李玉苓, 郭文征, 张卫民, 孙韬, 赵彩彦

061001 河北省沧州市传染病医院中西医肝病科(李玉苓,郭文征,张卫民,孙韬); 河北医科大学第三医院感染病科（赵彩彦）

收稿日期:2016-09-29 出版日期:2018-03-10 发布日期:2018-03-19
通讯作者: 赵彩彦,E-mail: zhaocy2005@163.com
作者简介:李玉苓,女,33岁,硕士研究生,主治医师。主要从事病毒性肝炎的防治研究。E-mail:lyl_2010@163.com

Expression of vitamin D receptor in cancerous tissues of patients with hepatitis B virus-related hepatocellular carcinoma

Li Yuling, Guo Wenzheng, Zhang Weimin, et al

Department of Liver Disease,Infectious Disease Hospital,Cangzhou 061001,Hebei Province,China

Received:2016-09-29 Online:2018-03-10 Published:2018-03-19

摘要/Abstract

摘要： 目的探讨乙型肝炎病毒相关性肝细胞癌（HCC）组织维生素D受体（VDR）表达变化及其与癌旁肝硬化组织和正常组织的异同。方法取10例乙型肝炎病毒相关性HCC组织及其癌旁肝组织和4例正常肝组织,采用实时荧光定量PCR法检测组织VDR mRNA水平,采用免疫组织化学法检测组织VDR表达,采用Western blot法检测组织VDR蛋白表达。结果免疫组织化学法检测发现HCC组织、癌旁组织和正常肝组织均有VDR表达,主要在细胞质表达,而细胞核基本无表达;癌组织VDR mRNA为（2.77±0.30）,显著高于癌旁肝硬化组织的（1.62±0.21）或正常肝组织的【（1.57±0.19）,P<0.01】;Western blot分析发现,HCC组织VDR蛋白相对表达量为（1.15±0.57）,显著高于癌旁肝硬化组织的（1.02±0.25）或正常肝脏组织的【（0.37±0.11）,P<0.01】。结论 VDR可能参与了HCC的发生发展过程。

关键词: 肝细胞癌, 维生素D, 维生素D受体

Abstract: Objective To explore the expression of vitamin D receptor（VDR） in cancerous tissues of patients with hepatitis B virus （HBV）-related hepatocellular carcinoma （HCC）. Methods The relative levels of VDR mRNA in liver tissues was detected by real-time PCR. VDR expression were determined by immunohistochemical method, and the relative expression of VDR protein was detected by Western blot. Results Immunohistochemistry showed that cancerous tissues, para-cancerous tissues, normal liver tissues expressed VDR equally. VDR was mainly expressed in the cytoplasm, and there was almost no expression in the nucleus. The RT-PCR detection showed that VDR mRNA in cancerous tissues was（2.77±0.30）, significantly higher than that in the para- cancerous tissues（1.62±0.21） or normal liver tissues[（1.57±0.19）, P<0.01];Western blot showed that the expression of VDR protein in cancerous tissues was（1.15±0.57）, significantly higher than that in the para- cancerous tissues（1.02±0.25） or in normal liver tissues[（0.37±0.11）, P<0.01]. Conclusion VDR is closely related to the development of hepatocellular carcinoma

Key words: Hepatoma, Vitamin D, Vitamin D receptor

李玉苓, 郭文征, 张卫民, 孙韬, 赵彩彦. 乙型肝炎病毒相关性肝癌组织维生素D受体表达情况分析[J]. 实用肝脏病杂志, 2018, 21(2): 249-252.

Li Yuling, Guo Wenzheng, Zhang Weimin, et al. Expression of vitamin D receptor in cancerous tissues of patients with hepatitis B virus-related hepatocellular carcinoma[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(2): 249-252.

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乙型肝炎病毒相关性肝癌组织维生素D受体表达情况分析

Expression of vitamin D receptor in cancerous tissues of patients with hepatitis B virus-related hepatocellular carcinoma

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