Abstract: Objective The purpose of this study was to investigate the application of indocyanine green(ICG) fluorescence imaging in laparoscopic cholecystectomy(LC) in the treatment of patients with benign gallbladder diseases. Methods 62 patients with benign gallbladder diseases, such as cholecystolithiasis and chronic cholecystitis, etc, were encountered in our hospital between October 2021 and July 2022, and were divided into two groups, with 31 cases in each, underwent LC under ICG fluorescent navigation or routine operation. Results The operation was successfully completed, and no iatrogenic biliary duct injury occurred in the two groups; one patient (3.2%)in the control group was transferred to open laparotomy; the time of identification and complete anatomic isolation of three tubes, operation time and intraoperative blood loss in the fluorescent navigation group were 0.5(0.5, 1)min, (16.9±0.4)min, 30(28, 33)min and 15(5, 20)ml, all significantly shorter or less than [2(1, 3)min, (24.9±0.1)min, 46(39, 53)min and 30(20, 40)ml, respectively, P< 0.05] in routine operation group; there was no significant difference respect to post-operational complications in the two groups (0.0% vs. 3.2%, P>0.05). Conclusion The ICG fluorescence navigation during LC is a very good measure, especially in difficult surgeries, which might be helpful to identify the anatomical structure of biliary duct, shorten operation times and reduce iatrogenic bile duct injury.

Key words: Cholecystolithiasis, Chronic cholecystitis, Laparoscopic cholecystectomy, Indocyanine green, Fluorescence navigation, Therapy